human bone marrow mesenchymal stem cells bmscs  (ATCC)

Journal: Biology

Article Title: Grape Pomace Polyphenolic Extract Promotes Osteogenic Differentiation in Human Mesenchymal Stem Cells Through Activation of RUNX2 and NRF2 Transcription Factors: A Potential Natural Strategy for Osteoporosis Prevention

doi: 10.3390/biology15090719

Figure Lengend Snippet: Effects of GPE on osteoblast differentiation of human MSCs. BMSCs and AdMSCs were cultured in basal medium (CTR) or osteogenic differentiation medium (OM) with or without GPE (1–10 µg GAE/mL) for up to 21 days, and matrix mineralization was analyzed by Alizarin Red S staining. Representative light microscopic images of Alizarin Red S staining in BMSCs ( A ) and AdMSCs ( B ) and quantitative analysis ( C ). Each experiment was performed in triplicate. All data are presented as mean ± standard deviation (SD). # p < 0.01 versus undifferentiated control cells (CTR); * p < 0.05 versus osteogenic differentiated cells (OM).

Article Snippet: Human bone marrow-derived mesenchymal stem cells (BMSCs) from healthy donors (ATCC-PCS-500-012) were purchased from ATCC (Milan, Italy) as well as mesenchymal stem cell basal medium (ATCC PCS500030) and mesenchymal stem cell growth kit for BMSC (ATCC PCS500041).

Techniques: Cell Culture, Staining, Standard Deviation, Control

Journal: Biology

Article Title: Grape Pomace Polyphenolic Extract Promotes Osteogenic Differentiation in Human Mesenchymal Stem Cells Through Activation of RUNX2 and NRF2 Transcription Factors: A Potential Natural Strategy for Osteoporosis Prevention

doi: 10.3390/biology15090719

Figure Lengend Snippet: Effects of GPE on the adipogenic differentiation of human MSCs. BMSCs and AdMSCs were cultured in basal medium (CTR) or adipogenic differentiation medium (AM) with or without GPE (1–10 µg GAE/mL) for up to 21 days, and lipid droplet formation was analyzed by Oil Red O (ORO) staining. Representative light microscopic images of ORO staining in BMSCs ( A ) and AdMSCs ( B ) and quantitative analysis ( C ). Each experiment was performed in triplicate. All data are presented as mean ± standard deviation (SD). # p < 0.01 versus undifferentiated control cells (CTR); * p < 0.05 and ** p < 0.01 versus adipogenic differentiated cells (AM).

Article Snippet: Human bone marrow-derived mesenchymal stem cells (BMSCs) from healthy donors (ATCC-PCS-500-012) were purchased from ATCC (Milan, Italy) as well as mesenchymal stem cell basal medium (ATCC PCS500030) and mesenchymal stem cell growth kit for BMSC (ATCC PCS500041).

Techniques: Cell Culture, Staining, Standard Deviation, Control

Journal: Journal of Materials Science. Materials in Medicine

Article Title: Copper tannic acid coordination nanosheet as a potent in-situ antibiotic sustained-release carrier for chronic osteomyelitis

doi: 10.1007/s10856-025-06979-z

Figure Lengend Snippet: The effect of Van@CuTA on BMSCs and HUVECs. A CCK-8 assay demonstrated no toxicity of Van@CuTA toward BMSCs. B Representative images of alizarin red staining, revealing increased calcium deposition in BMSCs treated with Van@CuTA. C , D Representative images and statistical analysis of HUVECs migration assay demonstrating a significant increase in migrated cells following Van@CuTA treatment. E – G Tube formation assay images and corresponding quantifications of total tube length and node number per view indicated that Van@CuTA significantly promoted HUVECs’ tube formation. (* indicated p < 0.05)

Article Snippet: Primary bone marrow mesenchymal stem cells (BMSCs) and Human Umbilical Vein Endothelial Cells (HUVECs) were obtained from ATCC.

Techniques: CCK-8 Assay, Staining, Migration, Tube Formation Assay

Journal: Materials Today Bio

Article Title: Umbilical cord blood-derived exosomes deliver miR-182-5p to Therapeutically target the MYD88/NF-κB signaling pathway in rat peri-implantitis

doi: 10.1016/j.mtbio.2025.102246

Figure Lengend Snippet: UCB-Exos promoted the proliferation and migration of BMSCs and exerted anti-inflammatory effects in vitro. (A) The cellular internalization of UCB-Exos by BMSCs. UCB-Exos were labeled with PKH-26 (red), and the nuclei of BMSCs were stained with Hoechst 33342 (blue). Scale bar = 20 μm. (B) The effect of UCB-Exos on the proliferation of BMSCs was detected by the CCK-8 assay. (C) The effect of UCB-Exos on the migration of BMSCs was detected by the cell scratch assay. Scale bar = 200 μm. (D) Quantitative analysis of migration rates. (E) The cellular internalization of UCB-Exos by RAW264.7. UCB-Exos were labeled with PKH-26 (red), and the nuclei of RAW264.7 were stained with Hoechst 33342 (blue). Scale bar = 20 μm. (F – H) Following 24-h co-culture of UCB-Exos with LPS-stimulated RAW264.7 cells, the gene expression of pro-inflammatory cytokines (TNF-α and IL-1β) and the anti-inflammatory cytokine (IL-10) was assessed by qRT-PCR. (I – K) ELISA detection of inflammatory factor (TNF-α, IL-1β and IL-10) concentration in the supernatant of RAW264.7 cells. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001; ns, no significance. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Human bone marrow mesenchymal stem cells (BMSCs) were obtained from Procell Life Science & Technology Co., Ltd (Wuhan, China).

Techniques: Migration, In Vitro, Labeling, Staining, CCK-8 Assay, Wound Healing Assay, Co-Culture Assay, Gene Expression, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Concentration Assay

Journal: Materials Today Bio

Article Title: Umbilical cord blood-derived exosomes deliver miR-182-5p to Therapeutically target the MYD88/NF-κB signaling pathway in rat peri-implantitis

doi: 10.1016/j.mtbio.2025.102246

Figure Lengend Snippet: UCB-Exos promoted osteogenic differentiation of BMSCs. (A) Calcium nodule formation (using ARS staining) was assessed at days 14 and 21. scale bar = 100 μm. (B) ALP expression was assessed at days 3 and 7 (using ALP staining). scale bar = 100 μm. (C – E) Expression of osteogenic genes (RUNX2, ALP, OPN) in BMSCs for 14 days after induction. (F) Expression of osteogenic proteins (RUNX2, ALP, OPN) in BMSCs for 7 and 14 days after induction. (G – I) Quantitative analysis of gray value in Western blot. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001; ns, no significance.

Article Snippet: Human bone marrow mesenchymal stem cells (BMSCs) were obtained from Procell Life Science & Technology Co., Ltd (Wuhan, China).

Techniques: Staining, Expressing, Western Blot

Journal: Materials Today Bio

Article Title: Umbilical cord blood-derived exosomes deliver miR-182-5p to Therapeutically target the MYD88/NF-κB signaling pathway in rat peri-implantitis

doi: 10.1016/j.mtbio.2025.102246

Figure Lengend Snippet: Characterization of the nGA composite hydrogels. (A) The nGA composite hydrogel was cured after photocrosslinking. (B) FTIR was used to characterize the chemical properties of the nGA composite hydrogel. GelMA, AlgMA, and GelMA/AlgMA were analyzed at intervals between 400 and 4000 cm −1 (C) The microstructure of GA composite hydrogel was observed by SEM. Scale bar = 10 μm. (D) Swelling and degradation (wet weight method) experiments determined the swelling and degradation performance of nGA and nGA-Exos at days 1, 2, 3, 6, 9, 12, 15, 18, and 21 (blue regions indicate degradation). Degradation of the composite hydrogels detected by dry weight method is shown in Fig. S1C. (E) The cumulative release rate of UCB-Exos released from the nGA-composite hydrogel was measured on days 1, 2, 3, 6, 9, 12, 15, 18, and 21. (F) Rheological testing examined the flow properties of the composite hydrogel, with the blue region indicating exposure to 405 nm blue light. (G) Compressive strain curves of the composite hydrogels. (H) A Live/dead staining of BMSCs on the hydrogel after days 2 of incubation. Scale bar = 200 μm. (I) The effect of nGA-Exos on the proliferation of BMSCs was detected by the CCK-8 assay. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001; ns, no significance. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Human bone marrow mesenchymal stem cells (BMSCs) were obtained from Procell Life Science & Technology Co., Ltd (Wuhan, China).

Techniques: Staining, Incubation, CCK-8 Assay

Journal: Materials Today Bio

Article Title: Umbilical cord blood-derived exosomes deliver miR-182-5p to Therapeutically target the MYD88/NF-κB signaling pathway in rat peri-implantitis

doi: 10.1016/j.mtbio.2025.102246

Figure Lengend Snippet: UCB-Exos deliver miR-182-5p targeting MYD88. (A) DEGs volcano plot, |log2FoldChange| > 1 and P-value<0.05. (B) DEGs heat map. (C) Analysis of significantly upregulated miRNA expression. (D) qRT-PCR validation of miR-182-5p expression in BMSCs of UCB-Exos treatment. (E) Specific site of interaction between miR-182–5p and MYD88 as predicted by the TargetScan website. (F) Dual luciferase reporter gene assay was used to verify the interaction between miR-182-5p and MYD88-3′UTR. (G – H) Western blotting was used to detect the protein expression of MYD88 in BMSCs after transfection with miR-182-5p inhibitors, NC-inhibitors, miR-182-5p mimics, and NC-mimics for 24 h. (I) qRT-PCR was used to detect the expression of MYD88 gene in BMSCs after transfection with miR-182-5p inhibitors, NC-inhibitors, miR-182-5p mimics, and NC-mimics for 24 h ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001; ns, no significance.

Article Snippet: Human bone marrow mesenchymal stem cells (BMSCs) were obtained from Procell Life Science & Technology Co., Ltd (Wuhan, China).

Techniques: Expressing, Quantitative RT-PCR, Biomarker Discovery, Luciferase, Reporter Gene Assay, Western Blot, Transfection

Journal: Materials Today Bio

Article Title: Umbilical cord blood-derived exosomes deliver miR-182-5p to Therapeutically target the MYD88/NF-κB signaling pathway in rat peri-implantitis

doi: 10.1016/j.mtbio.2025.102246

Figure Lengend Snippet: UCB-Exos promoted osteogenic differentiation of BMSCs via the delivery of miR-182-5p, which targeted and suppressed the MYD88/NF-κB signaling pathway. (A – B) Genes Expression of MYD88 and P65 in NC group, Exos + NC group and Exos + miR-182-5p inhibitors group. (C – E) Protein expression of MYD88, P-P65 and P65 in NC group, Exos + NC group and Exos + miR-182-5p inhibitors group. (F – H) Expression of osteogenic genes (RUNX2, ALP, OPN) in BMSCs for 7 days after induction. (I – L) Expression of osteogenic proteins (RUNX2, ALP, OPN) in BMSCs for 7 days after induction. (M) ALP expression was assessed at day 7 (using ALP staining). (N) Calcium nodule formation (using ARS staining) was assessed at day 14. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001; ns, no significance.

Article Snippet: Human bone marrow mesenchymal stem cells (BMSCs) were obtained from Procell Life Science & Technology Co., Ltd (Wuhan, China).

Techniques: Expressing, Staining